Setup of Microaquarium

I used the water sample from Meads Quarry, Island Home Ave,Knox Co. Tennessee Partial shade exposure Rock Quarry N35 57.162 W83 51.960 880 10/9/2011. When I observed the water sample in my tank I noticed one or two small swimming organisms. They would dart across the tank until they hit the wall. I took a sample of the mud, a sample of the middle layer of water, and a sample of the top of the water. This will give the organisms that live in the different zones a chance to thrive.